Nine US AI Startups Securing Over $100M in Funding in 2025: A New Era of Innovation

The artificial intelligence landscape in the United States is witnessing a remarkable surge, with US AI startups securing major funding in 2025 at an unprecedented pace. Here’s a detailed look at the nine US-based AI companies that have raised funding rounds of $100 million or more in the first quarter of 2025.

The AI Funding Boom: A 2025 Perspective

The year 2024 was a landmark for AI funding, with 49 startups raising $100 million or more. However, 2025 is already on track to potentially surpass this milestone. In just the first few months, nine companies have joined the exclusive “$100 million club,” highlighting the continued investor confidence in AI technologies. This trend is part of a broader AI funding boom that reflects the high costs and significant potential of AI development.

March: Anthropic’s Monumental Raise

Anthropic kicked off March with a staggering $3.5 billion Series E round, valuing the AI research and large language model company at $61.5 billion. This raise, led by Lightspeed with participation from industry giants like Salesforce Ventures and General Catalyst, underscores the immense potential seen in advanced AI development. This funding round is one of the largest in 2025, setting a high bar for US AI startups securing major funding.

February: A Month of AI Breakthroughs

February proved to be a particularly active month for AI funding:

Together AI secured a $305 million Series B, reaching a $3.3 billion valuation. This company is known for its open-source generative AI and infrastructure for AI model development.
Lambda, an AI infrastructure company, raised $480 million in a Series D round, valuing it at nearly $2.5 billion. This funding was co-led by SGW and Andra Capital, with participation from Nvidia and other investors.
Abridge, focusing on AI-powered medical transcription, closed a $250 million Series D at a $2.75 billion valuation. This Pittsburgh-based company is making significant strides in healthcare AI.
Eudia, an AI legal tech startup, impressed with a $105 million Series A. This funding round was led by General Catalyst and included participation from Floodgate, Defy Ventures, and Everywhere Ventures.
EnCharge AI raised $100 million in Series B funding for its innovative AI hardware solutions. Tiger Global led this round, with contributions from Scout Ventures, Samsung Ventures, and RTX Ventures.
Harvey, another AI legal tech company, secured $300 million in a Series D round, reaching a $3 billion valuation. This funding was led by Sequoia Capital and included investments from OpenAI Startup Fund, Kleiner Perkins, and Elad Gil.

January: Starting the Year Strong

The year began with two significant funding rounds:

ElevenLabs, a synthetic voice startup, raised $180 million in Series C funding, valuing the company at over $3 billion. This round was co-led by ICONIQ Growth and Andreessen Horowitz, with participation from Sequoia Capital and NEA.
Hippocratic AI, developing large language models for healthcare, announced a $141 million Series B round, reaching a valuation exceeding $1.6 billion. This funding was led by Kleiner Perkins, with participation from Andreessen Horowitz, Nvidia, and General Catalyst.

The Impact of Mega-Funding on AI Innovation

These substantial funding rounds are not just numbers; they represent a significant vote of confidence in the potential of US AI startups securing major funding. From healthcare and legal tech to infrastructure and voice synthesis, these startups are pushing the boundaries of what’s possible with artificial intelligence. The high costs associated with AI development, including hardware, staffing, and energy needs, are being met with widespread investor enthusiasm.

Looking Ahead: The Future of AI Funding

As we progress through 2025, it’s clear that investor appetite for AI startups remains strong. This trend suggests we may see even more groundbreaking developments and potentially more companies joining the $100 million club in the coming months. The focus is shifting towards sustainable growth and profitability, with investors becoming more selective and favoring companies with solid fundamentals and proven business models.

Regulatory and Market Dynamics

The investment landscape in 2025 is also influenced by regulatory concerns. Governments worldwide are ramping up efforts to regulate AI technologies to address issues such as data privacy, algorithmic bias, and security risks. This includes discussions about mandating audits of high-risk AI systems and potentially introducing liability rules for AI-generated content. These evolving regulatory landscapes are contributing to market unpredictability, making it crucial for startups to navigate complex regulatory environments.

The future of AI funding is marked by increased due diligence and a more calculated approach, reflecting a growing emphasis on navigating these complex regulatory landscapes. Despite these challenges, the enthusiasm for US AI startups securing major funding remains high, driven by the transformative potential of AI technologies across various industries.

Additional Resources:
https://www.cbinsights.com/research/report/ai-trends-2025/
https://www.natlawreview.com/article/state-funding-market-ai-companies-2024-2025-outlook
https://www.ycombinator.com/companies/industry/ai